Dynaflow® Resolve

The Dynaflow® Resolve system is an add-on to your existing patch-clamp setup that offers solution exchange at unsurpassed speed, control, and flexibility. The system allows for recording of any ion channel current, using any cell type, any compound, and any experiment. The system is used with the new Dynaflow® Resolve chip which has 16 channels and is made of glass. The chip is reusable and developed based on customer requirements reducing all risk for non-specific binding of “sticky compounds” and offering improved cost-efficiency.

Features

The Dynaflow® Resolve System has an extremely broad application base with regard to both cell types and ion channels. The flexibility of the Dynaflow® Resolve System along with the stability of experimental parameters makes it an ideal platform for conducting advanced patch-clamp experiments. The new and improved Dynaflow® Resolve system retains all the advantages from the well validated Dynaflow® System and adds the Resolve chip, providing reusability and enabling the use of sticky compounds. Dynaflow® Resolve System drastically reduces experimental set-up and allows for reproducible execution of precise and robust patch-clamp experiments with minimal compound consumption. The system includes the reusable microfluidic Resolve chip, a computer controlled scan stage used to translate chip movements, software to control and pre-program the movements of the scan stage, and a pump to drive the liquid flow.

Benefits

  • Any cell-type, any ion channel, any molecule
  • Adsorption-free perfusion of any compound
  • Programmable fast solution exchange – run complex perfusion protocols
  • Maximized data from each cell – from complete dose-responses to advanced receptor studies
  • Cost-efficiency – reusable Resolve chip and minimized compound and buffer consumption

Assays

  • Characterization of fast activating and desensitizing ion channels (AMPA, nACh)
  • Patch clamp-based fast synaptic transmission assays
  • Primary cell-based patch-clamp assays
  • Detailed analysis of ion channel kinetics

Resources

Publications

Glycine/Serine Polymorphism at Position 38 Influences KCNE1 Subunit’s Modulatory Actions on Rapid and Slow Delayed Rectifier K+ Currents
Author(s):

Yoshiaki Yamaguchi (1), Kohki Nishide (2), Mario Kato (2), Yukiko Hata (3), Koichi Mizumaki (4), Koshi Kinoshita (3), Yuki Nonobe (2), Toshihide Tabata (2), Tamotsu Sakamoto (1), Naoya Kataoka (1), Yosuke Nakatani (1), Fukiko Ichida (5), Hisashi Mori (6), Kenkichi Fukurotani (2), Hiroshi Inoue (1), Naoki Nishida (3)

1) Second Department of Internal Medicine, University of Toyama, Japan

2) Laboratory for Neural Information Technology, Graduate School of Sciences and Engineering, University of Toyama, Japan

3) Department of Legal Medicine, University of Toyama, Japan

4) Clinical Research and Ethics Center, University of Toyama, Japan

5) Department of Pediatrics, University of Toyama, Japan

6) Department of Molecular Neurosciences, University of Toyama, Japan

Published in: Circulation Journal, Vol. 78 (2014) No. 3 610-618

 

Sub-anesthetic concentrations of (R,S)-ketamine metabolites inhibit acetylcholine-evoked currents in α7 nicotinic acetylcholine receptors
Author(s):

Ruin Moaddel (1), Galia Abdrakhmanova (2), Joanna Kozak (3, 4), Krzysztof Jozwiak (3), Lawrence Toll (5), Lucita Jimenez (6), Avraham Rosenberg (1),Thao Tran (7), Yingxian Xiao (7), Carlos A. Zarate (8), Irving W. Wainer (1)

1) Laboratory of Clinical Investigation, Division of Intramural Research Programs, National institute on Aging, National Institutes of Health, Baltimore, MD, USA

2) Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA, USA

3) Laboratory of Medicinal Chemistry and Neuroengineering, Medical University of Lublin, Lublin, Poland

4) Department of Anatomy, Medical University of Lublin, Lublin, Poland

5) Torrey Pines Institute for Molecular Studies, Port St. Lucie, FL, USA

6) SRI International, Menlo Park, CA, USA

7) Department of Pharmacology and Physiology, Georgetown University, Washington, DC, USA

8) Experimental Therapeutics & Pathophysiology Branch, Division of Intramural Research Programs, National Institute of Mental Health, National Institutes of Health, Bethesda, MD, USA

Published in: European Journal of Pharmacology, Volume 698, Issues 1–3, (2013), Pages 228–234

 

A method for bidirectional solution exchange – “Liquid bullet” applications of acetylcholine to a7 nicotinic receptors
Author(s):

Nikolai Fedorov (1), Lisa Benson (1), John D. Graef (1), Jeremy Hyman (1), Jill Sollenberger (1), Fredrik Pettersson (2), Patrick M. Lippiello (1), Merouane Bencherif (1)

1) Targacept, Inc., Preclinical Research Department, 200 East First Street, Suite #300, Winston Salem, NC 27101, USA

2) Veprox AB, Hängpilsgatan 6, SE-426 77 Västra Frölunda, Sweden

Published in: Journal of Neuroscience Methods, Volume 206, Issue 1, 30 April 2012, Pages 23–33

 

Alternatively spliced domains interact to regulate BK potassium channel gating
Author(s):

Brandon E. Johnson (1), Dominique A. Glausern (1), Elise S. Dan-Glauser (2), D. Brent Halling (3), Richard W. Aldrich (3) and Miriam B. Goodman (1).

1) Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA, USA

2) Department of Psychology, Stanford University, Stanford, CA, USA

3) Section of Neurobiology, Center for Learning and Memory, University of Texas, Austin, TX, USA

Published in: PNAS December 20, 2011 vol. 108 no. 51 20784-20789

 

AF-353, a novel, potent and orally bioavailable P2X3/P2X2/3 receptor antagonist
Author(s):

Joel R Gever(1), Rothschild Soto (1), Robert A Henningsen (1), Renee S Martin (1), David H Hackos (1), Sandip Panicker (1), Werner Rubas (2), Ian B Oglesby (3), Michael P Dillon (4), Marcos E Milla (1), Geoffrey Burnstock (5) and Anthony PDW Ford (6)

1) Department of Inflammation Discovery, Roche Palo Alto, Palo Alto, CA, USA

2) Department of Metabolism and Pharmacokinetics, Roche Palo Alto, Palo Alto, CA, USA

3) Department of Neuroscience, Roche Palo Alto, Palo Alto, CA, USA

4) Department of Medicinal Chemistry, Roche Palo Alto, Palo Alto, CA, USA

5) Autonomic Neuroscience Centre, Royal Free and University College Medical School, London, UK

6) Afferent Pharmaceuticals, San Mateo, CA, USA

Published in: Br J Pharmacol. 2010 Jul;160(6):1387-98

 

Arrangement of Kv1 alpha subunits dictates sensitivity to tetraethylammonium
Author(s):

Al-Sabi A, Shamotienko O, Dhochartaigh SN, Muniyappa N, Le Berre M, Shaban H, Wang J, Sack JT, Dolly JO.

International Centre for Neurotherapeutics, Dublin City University, Dublin 9, Ireland.

Published in: J Gen Physiol. 2010 Sep;136(3):273-82.

 

Novel Alpha-7 Nicotinic Acetylcholine Receptor Agonists Containing a Urea Moiety: Identification and Characterization of the Potent, Selective, and Orally Efficacious Agonist 1-[6-(4-Fluorophenyl)pyridin-3-yl]-3-(4-piperidin-1-ylbutyl) Urea (SEN34625/WYE-103914)
Author(s):

Chiara Ghiron(1) et al;

(1) Siena Biotech SpA, Strada del Petriccio e Belriguardo 35, 53100 Siena, Italy

(2) Chemical Sciences and Neuroscience Discovery Research, Wyeth Research, CN 8000, Princeton, New Jersey 08543-8000

Published in: J. Med. Chem., 2010, 53 (11), pp 4379–4389 DOI: 10.1021/jm901692q

 

Telithromycin blocks neuromuscular transmission and inhibits nAChR currents in vitro
Author(s):

Chang-Ning Liu, Chris J. Somps

Department of Investigative Toxicology, Drug Safety Research & Development, Pfizer Global R&D, Groton, CT 06340, USA

Published in: Toxicol Lett. 2010 May 4;194(3):66-9. Epub 2010 Feb 12.

 

MEC-2 and MEC-6 in the Caenorhabditis elegans Sensory Mechanotransduction Complex: Auxiliary Subunits that Enable Channel Activity
Author(s):

Austin L. Brown(1), Zhiwen Liao(2), and Miriam B. Goodman(1,2)

1) Biophysics Program and 2) Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305

Published in: J. Gen. Physiol., Jun 2008; 131: 605 – 616.

 

Differential Structure of Atrial and Ventricular KATP Atrial KATP Channels Require SUR1
Author(s):

Thomas P. Flagg(1); Harley T. Kurata(1); Ricard Masia(1); George Caputa(1); Mark A. Magnuson(2); David J. Lefer(3); William A. Coetzee(4); and Colin G. Nichols(1)

1) Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Mo; Department of Molecular Physiology 2) Biophysics, Vanderbilt University School of Medicine, Nashville, Tenn 3) Department of Medicine and Pathology, Albert Einstein College of Medicine, New York; 4) Department of Pediatrics, New York University School of Medicine.

Published in: Circulation Research. 2008 Published online before print October 30, 2008, doi: 10.1161/CIRCRESAHA.108.17818

 

Role of cAMP sensor Epac as a determinant of K-ATP channel ATP-sensitivity in human pancreatic beta cells and rat INS-1 cells
Author(s):

Guoxin Kang, Colin A Leech, Oleg G Chepurny, William A Coetzee, and George G Holz

New York University School of Medicine

Published in: Kang et al. J Physiol.2008; 0: jphysiol.2007.143818v1

 

Gain-of-Function Mutations in the MEC-4 DEG/ENaC Sensory Mechanotransduction Channel Alter Gating and Drug Blockade
Author(s):

Austin L. Brown(1), Silvia M. Fernandez-Illescas(2), Zhiwen Liao2, and Miriam B. Goodman(1,2)

1) Biophysics Program and 2) Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305

Published in: J. Gen. Physiol., Jan 2007; 129: 161 – 173.

 

Controlling Desensitized States in Ligand-Receptor Interaction Studies with Cyclic Scanning Patch-Clamp Protocols
Author(s):

Daniel Granfeldt(1), Jon Sinclair(2), Maria Millingen(1), Cecilia Farre(2), Per Lincoln(1) and Owe Orwar(1)

1) Department of Chemical and Biological Engineering, Physical Chemistry, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Cellectricon AB, Fabriksgatan 7, SE-412 50 Göteborg, Sweden

Published in: Anal. Chem.; 2006; ASAP Article; DOI: 10.1021/ac060812z

 

A Biohybrid Dynamic Random Access Memory
Author(s):

Jon Sinclair(1), Daniel Granfeldt(1), Johan Pihl(2), Maria Millingen(1), Per Lincoln(1), Cecilia Farre(2), Lena Peterson(3), and Owe Orwar(1)

1) Department of Chemistry and Bioscience and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Cellectricon AB, Fabriksgatan 7, SE-412 50 Göteborg, Sweden 3) Department of Signal and Systems, Chalmers University of Technology, SE-412 96 Göteborg, Sweden

Published in: J. Am. Chem. Soc.; 128(15),5109-5113 (doi:10.1021/ja0580993), (2006)

 

An isoflurane- and alcohol-insensitive mutant GABAA receptor 1 subunit with near normal apparent affinity for GABA: characterization in heterologous systems and production of knock-in mice.
Author(s):

Cecilia M. Borghese (1), David F. Werner (2), Norbert Topf (3), Nicole V. Baron (3), L. Ashley Henderson (4), Stephen L. Boehm II (5), Yuri A. Blednov (4), Abdallah Saad (6), Shuiping Dai (6), Robert A. Pearce (6), R. Adron Harris (1), Gregg E. Homanics (2), Neil L. Harrison(3)

1) University of Texas – Austin 2) University of Pittsburgh 3) Weill Medical College of Cornell University 4) University of Texas at Austin 5) State University of New York – Binghamton 6) University of Wisconsin – Madison

Published in: J. Pharmacol. Exp. Ther. 2006: jpet.106.104406v1

 

Blocking Characteristics of hERG, hNav1.5, and hKvLQT1/ hminK after Administration of the Novel Anti-Arrhythmic Compound AZD7009
Author(s):

Frida Persson, M.Sc.,Leif Carlsson, Ph.D., Göran Duker, Ph.D., and Ingemar Jacobson, Ph.D.

AstraZeneca R&D Mölndal, Mölndal, Sweden

Published in: J Cardiovasc Electrophysiol, 16(3), 329-341,(2005)

 

Microfluidic device for creating gradients
Author(s):

Rajendrani Mukhopadhyay

Published in: Anal. Chem. A-Pages ; 2005; 77(13); 245A-245A.

 

A chemical waveform synthesizer
Author(s):

Jessica Olofsson(1), Helen Bridle(1), Jon Sinclair(1), Daniel Granfeldt(1), Eskil Sahlin(2), and Owe Orwar(1)

1) Department of Chemistry and Bioscience and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Department of Chemistry, Göteborg University, SE-412 96 Göteborg, Sweden

Edited by Richard N. Zare, Stanford University, Stanford, CA

Published in: PNAS; 102(23), 8097-8102, (doi:10.1073/pnas.0500230102), (2005)

 

Microfluidic technologies in drug discovery
Author(s):

Johan Pihl, Mattias Karlsson, Daniel T. Chiu

Published in: Drug Discovery Today, Vol 10, Number 20, October 2005

 

Patch clamp electrophysiology steps up a gear
Author(s):

Derek J. Trezise, Assay & Compound Profiling, GlaxoSmithKline Research & Development

Published in: European Pharmaceutical review, Issue 2, 2005

 

Microfluidic Gradient Generating Device for Pharmacological Profiling
Author(s):

Johan Pihl(1), Jon Sinclair(1), Eskil Sahlin(2), Mattias Karlsson(3), Fredrik Petterson(3), Jessica Olofsson(1), and Owe Orwar(1)

1) Department of Chemistry and Bioscience, and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Department of Chemistry, Göteborg University, SE-412 96 Göteborg, Sweden 3) Cellectricon AB, Fabriksgatan 7 SE-412 50 Göteborg, Sweden

Published in: Analytical Chemistry; 77(13), 3897-3903, (doi:10.1021/ac050218+), (2005)

 

Blocking characteristics of hKv1.5 and hKv4.3/hKChIP2.2 after administration of the novel antiarrhythmic compound AZD7009
Author(s):

Persson, Frida MSc; Carlsson, Leif PhD; Duker, Goran PhD; Ingemar Jacobson PhD

AstraZeneca R&D Mölndal, Mölndal, Sweden

Published in: Journal of Cardiovascular Pharmacology 46(1): 7-17. (2005).

 

Microfluidics and Patch-Clamp Based Sensors
Author(s):

Cecilia Farre, Mattias Karlsson, Jon Sinclair

Published in: Genetic Engineering News, Vol. 24, No 21, December 2004

 

A Microfluidics Approach to the Problem of Creating Separate Solution Environments Accessible from Macroscopic Volumes
Author(s):

Jessica Olofsson(1),Johan Pihl(1),Jon Sinclair(3),Eskil Sahlin(2), Mattias Karlsson(2), and Owe Orwar(1)

1)Department of Chemistry and Bioscience, and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden, 2) Cellectricon AB, Fabriksgatan 7 SE-412 50 Göteborg, Sweden, and 3) Department of Chemistry, Gothenburg University, SE-412 96 Göteborg, Sweden

Published in: Analytical Chemistry; 76(17), 4968 – 4976, (doi:10.1021/ac035527j), (2004)

 

Stabilization of High-Resistance Seals in Patch-Clamp Recordings by Laminar Flow
Author(s):

Jon Sinclair, Jessica Olofsson, Johan Pihl, and Owe Orwar

Department of Physical Chemistry and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden

Published in: Analytical Chemistry; 75(23), 6718-6722, (2003), (Technical Note)

 

Automated Electrophysiology: High Throughput of Art
Author(s):

Xiaobo Wang, Min Li

Published in: ASSAY and Drug Development Technologies. Oct 2003, Vol. 1, No. 5: 695-708

 

A Cell-Based Bar Code Reader for High-Throughput Screening of Ion Channel-Ligand Interactions
Author(s):

Jon Sinclair(1),Johan Pihl(1), Jessica Olofsson(1), Mattias Karlsson(1), Kent Jardemark(3), Daniel T. Chiu(2), and Owe Orwar(1)

1) Department of Physical Chemistry, and Microtechnology Centre, Chalmers University of Technology, SE-412 96 Göteborg, Sweden 2) Department of Chemistry, University of Washington, Seattle, Washington 98195-1700 3) Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden

Published in: Analytical Chemistry; Dec 15, 74(24), 6133-6138 (2002)

 

Screening of ion channel receptor agonists using capillary electrophoresis-patch clamp detection with resensitized detector cells
Author(s):

Cecilia Farre(1), Andreas Sjöberg(1), Kent Jardemark(1), Ingemar Jacobson(2), and Owe Orwar(1)

Department of Chemistry, Göteborg University, SE-412 96 Göteborg, Sweden, and AstraZeneca AB, SE-431 83 Mölndal, Sweden

Published in: Anal. Chem., Mar 15, 73(6), 1228-33 (2001)

Dynaflow® Resolve Add-On
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E-mail: info@novelscience.co.jp
Phone: +81 (0)44 221 3611
Web: http://www.novelscience.co.jp/